Coding

Part:BBa_K2686003:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-05)


Encapsulin protein -2 AA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 375
    Illegal SapI.rc site found at 406


Design Notes

The two amino acids were removed in order to liken the amino acid sequence from the end of the construct to the one found in literature.


Source

Genomic sequenced derived from a T. maritima plasmid from David Savage's lab.

References